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KMID : 0613820100200020275
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Journal of Life Science
2010 Volume.20 No. 2 p.275 ~ p.280
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Effect of Sulforaphane on LPS-Induced Matrix Metalloproteinase-9 (MMP-9) Expression
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Lee Jung-Tae
Woo Kyung-Jin
Kwon Taeg-Kyu
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Abstract
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Sulforaphane is a naturally occurring member of the iosothiocyanate family, which reveals chemopreventive capacities including anti-cancer, anti-inflammation and inhibition of MMP-9 activities. In this study, we investigated the effect of sulforaphane on the expression of matrix metalloproteinase-9 (MMP-9) in lipopolysaccharide (LPS)-induced Raw 264.7 cells. Sulforaphane strikingly suppressed the LPS-induced MMP-9 activity and mRNA expression in a dose-dependent manner. In addition, sulforaphane inhibited not only the LPS-induced MMP-9 promoter activity but also LPS-mediated activator protein-1 (AP-1) and nuclear factor-κB (NF-κB) promoter activity. Transient transfection by MMP-9 constructs, in which specific transcriptional factors were mutagenized, indicated that the effects of LPS and sulforaphane were mediated via AP-1 and NF-κB response elements. We found that sulforaphane had the ability to suppress LPS-induced invasion in vitro. Taken together, these results demonstrated that sulforaphane effectively suppressed LPS-induced MMP-9 expression via modulation of promoter elements (AP-1 and NF-κB) in MMP-9 transcriptional activation.
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KEYWORD
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Sulforaphane, matrix metalloproteinase-9(MMP-9), lipopolysaccharide (LPS), nuclear factor-κB(NF-κB), Raw 264.7 cells
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